Overview FlexVUE FixVUE U-VUE Contact

VUE panels for multiplex immunofluorescence

Overview

Characterize immune biology using pre-optimized panels and markers for whole slide analysis. Get unprecedented signal to noise, same slide H&E staining, fast turnaround time, and access to our team of assay development specialist guide you. With every solution we offer, Ultivue is setting the new standard for mIF solutions.

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FlexVUE

Quickly customize your immune profiling assay to discover the biology most relevant to your research questions. Starting with the Immuno8 FixVUE panel backbone, switch out up to 2 markers to create the 8-plex panel that identifies the immune cell subsets you need to find. Pre-optimized markers plus the robust InSituPlex technology means that you can customize without losing valuable time.

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FixVUE

(formerly UltiMapper® kits)

Choose from our pre-designed panels to identify specific cells in the tissue microenvironment. Get started quickly and assess the most interesting differences between tissue samples. Using InSituPlex technology, you can easily switch between panels and indications without adding timely re-optimization steps.

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U-VUE

When your assay requirements are unique, start with the extensive menu of pre-optimized U-VUE markers to build your panel. Fully customizable, and with our experts on hand to help guide your assay development, create a robust assay panel that will enable powerful spatial insights into the tissue microenvironment.

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Flexibility built for immune biology

The FlexVUE panel allows you to create a customized assay of 8 markers based on the Immuno8 FixVUE panel. Get a comprehensive view of the immune landscape or delve deeper into specific cell types and regions of the tissue microenvironment.

Illuminating
Biology

FlexVUE panel

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Exchange up to 2 markers from the list of pre-optimized markers. It’s that easy.

Create panels tailored to the biology you need. There’s a world of choices, click through to see just a few possibilities.

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Exchange up to 2 markers from the list of pre-optimized markers. It’s that easy.

Create panels tailored to the biology you need. There’s a world of choices, click through to see just a few possibilities.

photo

Exchange up to 2 markers from the list of pre-optimized markers. It’s that easy.

Create panels tailored to the biology you need. There’s a world of choices, click through to see just a few possibilities.

photo

Exchange up to 2 markers from the list of pre-optimized markers. It’s that easy.

Create panels tailored to the biology you need. There’s a world of choices, click through to see just a few possibilities.

photo

Exchange up to 2 markers from the list of pre-optimized markers. It’s that easy.

Create panels tailored to the biology you need. There’s a world of choices, click through to see just a few possibilities.


FlexVUE panels for custom biomarker discovery and analysis

  • Delivery in 4 weeks
  • Flexibly adapt your assay to support your hypothesis
  • Choose from a menu of pre-optimized markers
  • Add or subtract markers without assay redevelopment
  • Utilizes our robust 8-plex backbone
  • Same slide H&E staining
  • Co-register all images using UltiStacker software (included)


FlexVUE menu of markers

Exchange any two markers listed below with the markers in the
Immuno8 FixVUE panel:

 

Ki67 | GrzB | CD45RO | HLA-DR | CD56 | CD20 | CD11C | CD163 | CD206 | CD14
CD15 | CD11b | MHCII


Immuno8 FixVUE Panel

Characterize T-cell subsets, immunosuppressive cells and interactions along the PD-L1/PD-1 checkpoint axis with this comprehensive immuno-oncology focused panel. The Immuno8 FixVUE panel enables phenotyping of at least 14 distinct cell types in the tumor microenvironment. Use this panel to dive deeper into the biology or find the key differences between samples and scale down to the essential markers to move your science forward.

 

UltiStacker included All 4 and 8-plex panels include our new UltiStacker software to align your images accurately and easily. This essential tool brings together multiple fluorescent plus H&E images from any scanner to enable image comparison and analysis.

 

This antibody panel consists of the following markers:

 

CD3, CD4, CD8, CD68, FoxP3, PD-1, PD-L1, CK/SOX10, Nuclear counterstain

download pdf

Illuminating
Tissues

FixVUE Panels (formerly UltiMapper® kits)

Get started right away with a pre-configured 4- or 8-plex panel. Investigate a host of cell types and functions across multiple indications with the FixVUE ready-to-go panels shipped directly to your lab.
All our FixVUE and FlexVUE panels include our NEW UltiStacker software. To get the most out of your images, utilize UltiStacker software to align both fluorescent and H&E images from your assays.

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Determine whether the tumor is “hot” or “cold” with this 4-plex panel. Co-localize macrophages, cytotoxic T cells, tumor cells, and the underlying inhibitory or inflammatory mechanisms at play along the PD-L1 axis.

This antibody panel consists of the following markers:

CD8 | CD68 | PD-L1 | PanCK/SOX10 | Nuclear counterstain

download pdf

Identify regulatory T cells and cytotoxic T cells within the tumor context with this 4-plex panel. Characterize the different T cells that regulate immune response.

This antibody panel consists of the following markers:

CD4 | CD8 | FoxP3 | panCK/SOX10 | Nuclear counterstain

download pdf

Identify Myeloid Derived Suppressor Cells within the tumor microenvironment with this 4-plex panel. Using the antibody panel, characterize cell populations into M-MDSCs and PMN-MDSCs.

This antibody panel consists of the following markers:

CD11b | CD14 | CD15 | HLA-DR | Nuclear counterstain

download pdf

Determine whether the immune response has exhausted with this 4-plex panel. Co-localize T cells, memory T cells, tumor cells, and the expression of the underlying PD-1 checkpoint to decipher the immune exhaustion mechanism at play.

This antibody panel consists of the following markers:

CD3 | CD45RO | PD-1 | panCK/SOX10 | Nuclear counterstain

download pdf

Determine whether T cells have the potential to mediate cell death with this 4-plex panel. This T cell activation panel enables the co-expression of proliferating cells, cytotoxic cells, tumor cells, and the underlying proliferative index and potential for T cell mediated death.

This antibody panel consists of the following markers:

CD3 | Granzyme B | Ki67 | panCK/SOX10 | Nuclear counterstain

download pdf

Identify and characterize professional antigen-presenting cells (APCs) with this 4-plex panel. Visualize the co-expression of specific subsets of cells along the dendritic, monocyte/macrophage, and B cell lineages.

This antibody panel consists of the following markers:

CD11c | CD20 | CD68/CD163 | MHC Class II | Nuclear counterstain

download pdf

Partner with Ultivue to reduce multiplex assay development time, efficiently assess clinical utility and refine biomarker panels.

With a powerful assay, you have the knowledge in hand to make better decisions that can ultimately lead to more successful clinical utility.

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Develop customized biomarker panels to get the spatial insights that matter to you.

The U-VUE Biomarker menu consists of pre-optimized markers, ready to be built into multiplex panels. Assess cell phenotypes and marker expression, find tumor and stromal regions, and understand spatial relationships across the whole slide. Select the markers you need from the list, up to 8-plex, and create a custom solution to fit your specific analysis needs.

Not sure how to get the most out of your tissues? Our in-house team of experts can assist across the entire spectrum of your development and partner with you to find the custom solution to answer your biological question.

 

 

 

antibody selection and biomarker strategy: ensure that your design a panel optimized for the best data
pathological assessment: our pathologists can assist with tailoring your project to maximize its relevance
assay development: get assistance to ensure that your assay will translate from research to clinical use.
image and data analysis: computational expertise to find the key features and develop robust algorithms

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